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Discussion Autophagy is a dynamic process involving bulk degradation of portions of the cytoplasm and intracellular organelles in eukaryotic cells via the lysosomal system. It plays an important role in cellular homeostasis and is involved in a number of human diseases, including cancer, neurodegeneration, and brain ischemia [1, 8–10]. Our recent studies demonstrate that autophagy occurs in the brain after ICH [4]. After ICH, iron concentrations in the brain can reach very high levels and brain non-heme iron is not cleared from the brain within 4 weeks [15].

Song, W. Liu, R. F. Keep, G. Xi Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI, USA Summary Autophagy occurs in the brain after intracerebral hemorrhage (ICH). Iron is an important factor causing neuronal death and brain atrophy after ICH. In this study, we examined whether iron can induce autophagy in the hippocampus and in cultured neurons. For in vivo studies, rats received an infusion of either saline or ferrous iron into the right hippocampus and were killed 1, 3, or 7 days later for Western blot analysis of microtubule-associated protein light chain-3 (LC3).

Part V. Two groups of 3 rats each were examined in this part. All rats received a 30-mL infusion of bovine hemoglobin (300 mg=mL in saline), followed immediately by intraperitoneal injection of 1 mL saline or 500 mg=kg deferoxamine (dissolved in 1 mL saline). The rats were killed 24 h later for Western blot analysis. Measurement of brain water and ion content Animals were re-anesthetized 24 h later with 60 mg=kg pentobarbital administered intraperitoneally and killed by decapitation. Brains were removed and a 3-mm thick coronal brain slice was cut with a blade approximately 4 mm from the frontal pole.

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A Guide to the Solidification of Steels by Jernkontoret


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