New PDF release: Calcium Signaling Protocols - 2nd ed (Methods in Molecular

By David G. Lambert

ISBN-10: 1588294420

ISBN-13: 9781588294425

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Extra info for Calcium Signaling Protocols - 2nd ed (Methods in Molecular Biology)

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A. Devi, Department of Pharmacology, New York University, NY). 6. 5 µg/mL fungizone, and 100 µg/mL geneticin (see Notes 1 and 3). 2. Buffers 1. 4 titrated with 10M NaOH. 2. 1 mM EGTA. This should be made in plastic beakers as glass leaches significant amounts of Ca2+. 3. 7 mM glucose, and 10 mM HEPES. With this buffer, 100 mM K+ is added (see Note 4). 4. 4 (with 10 M NaOH). 3. General Reagents 1. Fura-2/AM (Sigma, Dorset, UK). Make up as a stock (1 mM) solution by dissolving in dimethylsulfoxide and storing aliquots (10 µL) at –20°C until required.

92. Nagai, T. , Park, E. , and Miyawaki, A. (2002). A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20, 87–90. 93. , et al. (2001) FRET-based in vivo Ca2+ imaging by a new calmodulin-GFP fusion molecule. Nat. Struct. Biol. 8, 1069–1073. 94. , Lynch, J. A. and Romoser, V. A. (1997) Novel fluorescent indicator proteins for monitoring free intracellular Ca2+. Cell Calcium 22, 209–216. 95. Black, D. , and Persechini, A. (2003) Monitoring the total available calmodulin conentration in intact cells over the physiological range in free Ca2+.

5 µg/mL fungizone, and 100 µg/mL geneticin (see Notes 1 and 3). 2. Buffers 1. 4 titrated with 10M NaOH. 2. 1 mM EGTA. This should be made in plastic beakers as glass leaches significant amounts of Ca2+. 3. 7 mM glucose, and 10 mM HEPES. With this buffer, 100 mM K+ is added (see Note 4). 4. 4 (with 10 M NaOH). 3. General Reagents 1. Fura-2/AM (Sigma, Dorset, UK). Make up as a stock (1 mM) solution by dissolving in dimethylsulfoxide and storing aliquots (10 µL) at –20°C until required. 2. Triton X-100 (Sigma).

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Calcium Signaling Protocols - 2nd ed (Methods in Molecular Biology) by David G. Lambert


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